{"id":6839,"date":"2026-06-22T13:38:53","date_gmt":"2026-06-22T11:38:53","guid":{"rendered":"https:\/\/mef.sum.ba\/sme\/?page_id=6839"},"modified":"2026-06-22T14:01:31","modified_gmt":"2026-06-22T12:01:31","slug":"laboratorij-za-primijenjenu-fiziologiju-i-farmakologiju","status":"publish","type":"page","link":"https:\/\/mef.sum.ba\/sme\/laboratorij-za-primijenjenu-fiziologiju-i-farmakologiju\/","title":{"rendered":"Laboratorij za primijenjenu fiziologiju i farmakologiju"},"content":{"rendered":"\t\t
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Laboratorij za primijenjenu fiziologiju i farmakologiju<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Laboratorij za izolirane organe<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Medicinski fakultet u Mostaru je prilikom opremanja laboratorija za temeljna medicinska istra\u017eivanja nabavio dva sustava za izolirane organe, sustav za izolirano srce \u201eprema Langendorffu\u201c i sustav za izolirane krvne \u017eile. Sustavi za izolirane organe nude brojne mogu\u0107nosti u bazi\u010dnim farmakolo\u0161kim, fiziolo\u0161kim i patofiziolo\u0161kim istra\u017eivanjim kardiovaskularnog sustava.<\/p>

Oscar Langendorff\u00a0 je 1897. godine opisao tehniku izolacije srca koje i izvan tijela nastavlja sa svojom aktivno\u0161\u0107u i pri tome je mogu\u0107e pratiti razli\u010dite parametre sr\u010dane funkcije. Model se zasniva na retrogradnoj perfuziji srca hranjivom otopinom kroz aortu i ostia coronaria u koronarne krvne \u017eile, \u0161to omogu\u0107uje du\u017ee izvantjelesno o\u010duvanje srca. Nepretkom tehnike sustav je modificiran i unaprije\u0111en, ali je izvorni princip funkcioniranja ostao nepromijenjen. Sustav za izolirano srce \u201eprema Langendorffu\u201c danas je standardan model u temeljnim farmakolo\u0161kim istra\u017eivanjima. Primjenjuje se za in vitro\u00a0 istra\u017eivanja izravnih u\u010dinaka razli\u010ditih tvari na parametre sr\u010dane funkcije u odsutnosti neuralnih i humoralnih \u010dimbenika. To zna\u010di da je svaka promjena sr\u010dane aktivnosti izravni u\u010dinak tvari koju primjenjujemo i nije \u201emaskirana\u201c kompenzatornim mehanizmima intaktnog organizma. Na sustavu za izolirano srce mogu se istra\u017eivati u\u010dinci razli\u010ditih tvari u bazalnim i uvjetima ishemije i reperfuzije. Uvjeti ishemije-reperfuzije vjerno opona\u0161aju uvjete infarkta miokarda pa se na njemu mogu tako\u0111er istra\u017eivati supstance koje su potencijalni kardioprotektori tijekom ishemije i\u00a0 reperfuzije. Na sustavu za izolirano srce koji imamo u Mostaru mogu se mjeriti slijede\u0107i parametri sr\u010dane funkcije: koronarni protok, sistoli\u010dki i dijastoli\u010dki tlak u lijevom ventrikulu, sra\u010danu frekvenciju, atrio-ventrikularno vrijeme provo\u0111enja, potro\u0161nju kisika te indirektno jo\u0161 brojni parametri.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Sustav za izolirane krvne \u017eile nudi brojne mogu\u0107nosti za istra\u017eivanje vazodilatacijskih u\u010dinaka i mehanizama razli\u010ditih vazoaktivnih supstanci. Izolirane krvne \u017eile (aorta, mezenterijalne arterije, koronarne arterije) se uzmu od pokusne \u017eivotinje i nare\u017eu na prstenove veli\u010dine 3-4 mm. Izolirani prstenovi se razapnu izme\u0111u dvije \u017eice, jedna je fiksirana za organski bazen\u010di\u0107, a druga je spojena sa pretvara\u010dem sile tlaka. Prstenovi se napregnu silom od 20 mN, stabiliziraju kroz 60 minuta, a nakon toga se prekontrahiraju noradrenalinom. Nakon \u0161to je postignut plato kontrakcije dodaje se acetilkolin koji slu\u017ei za procjenu o\u010duvanosti endotela krvne \u017eile. Relaksacija na acetilkolin mora biti ve\u0107e od 50% da bi \u017eile smatrali funkcionalnim. Nakon toga slijedi izvo\u0111enje planiranog pokusa i istra\u017eivanja potencijalno vazoaktivne supstance.<\/p>

Srce i krvne \u017eile uzimaju se od pokusnih \u017eivotinja kao \u0161to su mi\u0161evi, \u0161takori, zamor\u010di\u0107i ili kuni\u0107i. Izolirani organi se u in vitro uvjetima prehranjuju Krebs-Hensseleitovom otopinom koja je oksigenirana i zagrijana na temperaturu od 36,7 C.<\/p>

Osim u istra\u017eiva\u010dke svrhe, sustavi za izolirane organe mogu se koristiti i u nastavne svrhe. Studenti na vje\u017ebama iz farmakologije mogu \u201eu\u017eivo\u201c vidjeti u\u010dinke brojnih lijekova na srce i krvne \u017eile. Tako\u0111er se mogu pokazivati mehanizmi dijelovanja pojedinih lijekova. Tijekom nastave iz fiziologije studenti su u mogu\u0107nosti prou\u010davati promjene na srcu prilikom promjene koncentracije kisika, hranjivih tvari ili elektrolita u otopini kojom se hrani srce.<\/p>

Ovi sustavi predstavljaju novost u Mostaru i Bosni i Hercegovini te daju novu dimenziju znanstveno-istra\u017eiva\u010dkom radu na na\u0161em fakultetu. Tako\u0111er pru\u017eaju mogu\u0107nost studentima za izradu diplomskih radova i lije\u010dnicima za izvo\u0111enje pokusa za svoje magisterije i doktorate.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Suradnja\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Laboratorij za primijenjenu fiziologiju i farmakologiju<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSveu\u010dili\u0161na klini\u010dka bolnica Mostar\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tOdsjek invazivne kardiologije\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMedicinski fakultet u Splitu \t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKatedra za farmakologiju\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMedicinski fakultet u Osijeku\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKatedra za fiziologiju\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBiokemijski fakultet u Mariboru\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKlini\u010dka bolnica Split\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKlinika za zarazne bolesti\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t

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Objavljeni radovi zadnjih 5 godina:<\/strong><\/p>

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  2. Karabuva S, Brizi\u0107 I, Latinovi\u0107 Z, Leonardi A, Kri\u017eaj I, Luk\u0161i\u0107 B. Cardiotoxic effects of the Vipera ammodytes ammodytes venom fractions in the isolated perfused rat heart. Toxicon. 2016 Oct;121:98-104. doi: 10.1016\/j toxicon. 2016. 09. 001.<\/li>
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  7. Markota D<\/a>,\u00a0Markota I<\/a>,\u00a0Starcevic B<\/a>,\u00a0Tomic M<\/a>,\u00a0Prskalo Z<\/a>,\u00a0Brizic I<\/a>. Prevention of contrast-induced nephropathy with Na\/K citrate.\u00a0Eur Heart J.<\/a>\u00a02013;34(30):2362-7<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    Laboratorij za primijenjenu fiziologiju i farmakologiju Laboratorij za izolirane organe Medicinski fakultet u Mostaru je prilikom opremanja laboratorija za temeljna medicinska istra\u017eivanja nabavio dva sustava za izolirane organe, sustav za izolirano srce \u201eprema Langendorffu\u201c i sustav za izolirane krvne \u017eile. Sustavi za izolirane organe nude brojne mogu\u0107nosti u bazi\u010dnim farmakolo\u0161kim, fiziolo\u0161kim i patofiziolo\u0161kim istra\u017eivanjim kardiovaskularnog […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_angie_page":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"disabled","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"enabled","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-6839","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/pages\/6839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/comments?post=6839"}],"version-history":[{"count":46,"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/pages\/6839\/revisions"}],"predecessor-version":[{"id":6888,"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/pages\/6839\/revisions\/6888"}],"wp:attachment":[{"href":"https:\/\/mef.sum.ba\/sme\/wp-json\/wp\/v2\/media?parent=6839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}