{"id":6839,"date":"2026-06-22T13:38:53","date_gmt":"2026-06-22T11:38:53","guid":{"rendered":"https:\/\/mef.sum.ba\/sme\/?page_id=6839"},"modified":"2026-06-22T14:01:31","modified_gmt":"2026-06-22T12:01:31","slug":"laboratorij-za-primijenjenu-fiziologiju-i-farmakologiju","status":"publish","type":"page","link":"https:\/\/mef.sum.ba\/sme\/laboratorij-za-primijenjenu-fiziologiju-i-farmakologiju\/","title":{"rendered":"Laboratorij za primijenjenu fiziologiju i farmakologiju"},"content":{"rendered":"\t\t
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Laboratorij za primijenjenu fiziologiju i farmakologiju<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Laboratorij za izolirane organe<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Medicinski fakultet u Mostaru je prilikom opremanja laboratorija za temeljna medicinska istra\u017eivanja nabavio dva sustava za izolirane organe, sustav za izolirano srce \u201eprema Langendorffu\u201c i sustav za izolirane krvne \u017eile. Sustavi za izolirane organe nude brojne mogu\u0107nosti u bazi\u010dnim farmakolo\u0161kim, fiziolo\u0161kim i patofiziolo\u0161kim istra\u017eivanjim kardiovaskularnog sustava.<\/p>

Oscar Langendorff\u00a0 je 1897. godine opisao tehniku izolacije srca koje i izvan tijela nastavlja sa svojom aktivno\u0161\u0107u i pri tome je mogu\u0107e pratiti razli\u010dite parametre sr\u010dane funkcije. Model se zasniva na retrogradnoj perfuziji srca hranjivom otopinom kroz aortu i ostia coronaria u koronarne krvne \u017eile, \u0161to omogu\u0107uje du\u017ee izvantjelesno o\u010duvanje srca. Nepretkom tehnike sustav je modificiran i unaprije\u0111en, ali je izvorni princip funkcioniranja ostao nepromijenjen. Sustav za izolirano srce \u201eprema Langendorffu\u201c danas je standardan model u temeljnim farmakolo\u0161kim istra\u017eivanjima. Primjenjuje se za in vitro\u00a0 istra\u017eivanja izravnih u\u010dinaka razli\u010ditih tvari na parametre sr\u010dane funkcije u odsutnosti neuralnih i humoralnih \u010dimbenika. To zna\u010di da je svaka promjena sr\u010dane aktivnosti izravni u\u010dinak tvari koju primjenjujemo i nije \u201emaskirana\u201c kompenzatornim mehanizmima intaktnog organizma. Na sustavu za izolirano srce mogu se istra\u017eivati u\u010dinci razli\u010ditih tvari u bazalnim i uvjetima ishemije i reperfuzije. Uvjeti ishemije-reperfuzije vjerno opona\u0161aju uvjete infarkta miokarda pa se na njemu mogu tako\u0111er istra\u017eivati supstance koje su potencijalni kardioprotektori tijekom ishemije i\u00a0 reperfuzije. Na sustavu za izolirano srce koji imamo u Mostaru mogu se mjeriti slijede\u0107i parametri sr\u010dane funkcije: koronarni protok, sistoli\u010dki i dijastoli\u010dki tlak u lijevom ventrikulu, sra\u010danu frekvenciju, atrio-ventrikularno vrijeme provo\u0111enja, potro\u0161nju kisika te indirektno jo\u0161 brojni parametri.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Sustav za izolirane krvne \u017eile nudi brojne mogu\u0107nosti za istra\u017eivanje vazodilatacijskih u\u010dinaka i mehanizama razli\u010ditih vazoaktivnih supstanci. Izolirane krvne \u017eile (aorta, mezenterijalne arterije, koronarne arterije) se uzmu od pokusne \u017eivotinje i nare\u017eu na prstenove veli\u010dine 3-4 mm. Izolirani prstenovi se razapnu izme\u0111u dvije \u017eice, jedna je fiksirana za organski bazen\u010di\u0107, a druga je spojena sa pretvara\u010dem sile tlaka. Prstenovi se napregnu silom od 20 mN, stabiliziraju kroz 60 minuta, a nakon toga se prekontrahiraju noradrenalinom. Nakon \u0161to je postignut plato kontrakcije dodaje se acetilkolin koji slu\u017ei za procjenu o\u010duvanosti endotela krvne \u017eile. Relaksacija na acetilkolin mora biti ve\u0107e od 50% da bi \u017eile smatrali funkcionalnim. Nakon toga slijedi izvo\u0111enje planiranog pokusa i istra\u017eivanja potencijalno vazoaktivne supstance.<\/p>

Srce i krvne \u017eile uzimaju se od pokusnih \u017eivotinja kao \u0161to su mi\u0161evi, \u0161takori, zamor\u010di\u0107i ili kuni\u0107i. Izolirani organi se u in vitro uvjetima prehranjuju Krebs-Hensseleitovom otopinom koja je oksigenirana i zagrijana na temperaturu od 36,7 C.<\/p>

Osim u istra\u017eiva\u010dke svrhe, sustavi za izolirane organe mogu se koristiti i u nastavne svrhe. Studenti na vje\u017ebama iz farmakologije mogu \u201eu\u017eivo\u201c vidjeti u\u010dinke brojnih lijekova na srce i krvne \u017eile. Tako\u0111er se mogu pokazivati mehanizmi dijelovanja pojedinih lijekova. Tijekom nastave iz fiziologije studenti su u mogu\u0107nosti prou\u010davati promjene na srcu prilikom promjene koncentracije kisika, hranjivih tvari ili elektrolita u otopini kojom se hrani srce.<\/p>

Ovi sustavi predstavljaju novost u Mostaru i Bosni i Hercegovini te daju novu dimenziju znanstveno-istra\u017eiva\u010dkom radu na na\u0161em fakultetu. Tako\u0111er pru\u017eaju mogu\u0107nost studentima za izradu diplomskih radova i lije\u010dnicima za izvo\u0111enje pokusa za svoje magisterije i doktorate.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Suradnja\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Laboratorij za primijenjenu fiziologiju i farmakologiju<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSveu\u010dili\u0161na klini\u010dka bolnica Mostar\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tOdsjek invazivne kardiologije\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMedicinski fakultet u Splitu \t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKatedra za farmakologiju\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMedicinski fakultet u Osijeku\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKatedra za fiziologiju\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBiokemijski fakultet u Mariboru\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKlini\u010dka bolnica Split\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/h3>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tKlinika za zarazne bolesti\t\t\t\t\t<\/p>\n\t\t\t\t\n\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t

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Objavljeni radovi zadnjih 5 godina:<\/strong><\/p>

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  7. Markota D<\/a>,\u00a0Markota I<\/a>,\u00a0Starcevic B<\/a>,\u00a0Tomic M<\/a>,\u00a0Prskalo Z<\/a>,\u00a0Brizic I<\/a>. Prevention of contrast-induced nephropathy with Na\/K citrate.\u00a0Eur Heart J.<\/a>\u00a02013;34(30):2362-7<\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    Laboratorij za primijenjenu fiziologiju i farmakologiju Laboratorij za izolirane organe Medicinski fakultet u Mostaru je prilikom opremanja laboratorija za temeljna 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    Medicinski fakultet u Mostaru je prilikom opremanja laboratorija za temeljna medicinska istra\\u017eivanja nabavio dva sustava za izolirane organe, sustav za izolirano srce \\u201eprema Langendorffu\\u201c i sustav za izolirane krvne \\u017eile. Sustavi za izolirane organe nude brojne mogu\\u0107nosti u bazi\\u010dnim farmakolo\\u0161kim, fiziolo\\u0161kim i patofiziolo\\u0161kim istra\\u017eivanjim kardiovaskularnog sustava.<\\\/p>

    Oscar Langendorff\\u00a0 je 1897. godine opisao tehniku izolacije srca koje i izvan tijela nastavlja sa svojom aktivno\\u0161\\u0107u i pri tome je mogu\\u0107e pratiti razli\\u010dite parametre sr\\u010dane funkcije. Model se zasniva na retrogradnoj perfuziji srca hranjivom otopinom kroz aortu i ostia coronaria u koronarne krvne \\u017eile, \\u0161to omogu\\u0107uje du\\u017ee izvantjelesno o\\u010duvanje srca. Nepretkom tehnike sustav je modificiran i unaprije\\u0111en, ali je izvorni princip funkcioniranja ostao nepromijenjen. Sustav za izolirano srce \\u201eprema Langendorffu\\u201c danas je standardan model u temeljnim farmakolo\\u0161kim istra\\u017eivanjima. Primjenjuje se za in vitro\\u00a0 istra\\u017eivanja izravnih u\\u010dinaka razli\\u010ditih tvari na parametre sr\\u010dane funkcije u odsutnosti neuralnih i humoralnih \\u010dimbenika. To zna\\u010di da je svaka promjena sr\\u010dane aktivnosti izravni u\\u010dinak tvari koju primjenjujemo i nije \\u201emaskirana\\u201c kompenzatornim mehanizmima intaktnog organizma. Na sustavu za izolirano srce mogu se istra\\u017eivati u\\u010dinci razli\\u010ditih tvari u bazalnim i uvjetima ishemije i reperfuzije. Uvjeti ishemije-reperfuzije vjerno opona\\u0161aju uvjete infarkta miokarda pa se na njemu mogu tako\\u0111er istra\\u017eivati supstance koje su potencijalni kardioprotektori tijekom ishemije i\\u00a0 reperfuzije. Na sustavu za izolirano srce koji imamo u Mostaru mogu se mjeriti slijede\\u0107i parametri sr\\u010dane funkcije: koronarni protok, sistoli\\u010dki i dijastoli\\u010dki tlak u lijevom ventrikulu, sra\\u010danu frekvenciju, atrio-ventrikularno vrijeme provo\\u0111enja, potro\\u0161nju kisika te indirektno jo\\u0161 brojni parametri.<\\\/p>\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"95dae83\"}]},\"elements\":[],\"widgetType\":\"text-editor\"}],\"isInner\":false},{\"id\":\"8391c80\",\"elType\":\"column\",\"settings\":{\"_column_size\":50,\"_inline_size\":35,\"_inline_size_tablet\":40,\"margin_tablet\":{\"unit\":\"px\",\"top\":\"0\",\"right\":\"0\",\"bottom\":\"0\",\"left\":\"0\",\"isLinked\":false},\"margin_mobile\":{\"unit\":\"px\",\"top\":\"0\",\"right\":\"0\",\"bottom\":\"0\",\"left\":\"0\",\"isLinked\":false},\"padding_mobile\":{\"unit\":\"px\",\"top\":\"0\",\"right\":\"0\",\"bottom\":\"0\",\"left\":\"0\",\"isLinked\":false},\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"87ed54e\"}]},\"elements\":[{\"id\":\"81cafaa\",\"elType\":\"widget\",\"settings\":{\"image\":{\"id\":6853,\"url\":\"https:\\\/\\\/mef.sum.ba\\\/sme\\\/wp-content\\\/uploads\\\/2026\\\/06\\\/pexels-photo-7269622-7269622-scaled.jpg\",\"alt\":\"Flat lay of educational toy models of human organs for anatomy learning.\",\"source\":\"library\",\"size\":\"\"},\"image_size\":\"full\",\"image_custom_dimension\":{\"width\":\"600\",\"height\":\"840\"},\"image_border_radius\":{\"unit\":\"px\",\"top\":\"2\",\"right\":\"2\",\"bottom\":\"2\",\"left\":\"2\",\"isLinked\":true},\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"5c9d52d\"}]},\"elements\":[],\"widgetType\":\"image\"}],\"isInner\":false}],\"isInner\":false},{\"id\":\"6132c8e\",\"elType\":\"container\",\"settings\":{\"flex_direction\":\"column\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"c8d7e96\"}]},\"elements\":[{\"id\":\"edd2557\",\"elType\":\"widget\",\"settings\":{\"editor\":\"

    Sustav za izolirane krvne \\u017eile nudi brojne mogu\\u0107nosti za istra\\u017eivanje vazodilatacijskih u\\u010dinaka i mehanizama razli\\u010ditih vazoaktivnih supstanci. Izolirane krvne \\u017eile (aorta, mezenterijalne arterije, koronarne arterije) se uzmu od pokusne \\u017eivotinje i nare\\u017eu na prstenove veli\\u010dine 3-4 mm. Izolirani prstenovi se razapnu izme\\u0111u dvije \\u017eice, jedna je fiksirana za organski bazen\\u010di\\u0107, a druga je spojena sa pretvara\\u010dem sile tlaka. Prstenovi se napregnu silom od 20 mN, stabiliziraju kroz 60 minuta, a nakon toga se prekontrahiraju noradrenalinom. Nakon \\u0161to je postignut plato kontrakcije dodaje se acetilkolin koji slu\\u017ei za procjenu o\\u010duvanosti endotela krvne \\u017eile. Relaksacija na acetilkolin mora biti ve\\u0107e od 50% da bi \\u017eile smatrali funkcionalnim. Nakon toga slijedi izvo\\u0111enje planiranog pokusa i istra\\u017eivanja potencijalno vazoaktivne supstance.<\\\/p>

    Srce i krvne \\u017eile uzimaju se od pokusnih \\u017eivotinja kao \\u0161to su mi\\u0161evi, \\u0161takori, zamor\\u010di\\u0107i ili kuni\\u0107i. Izolirani organi se u in vitro uvjetima prehranjuju Krebs-Hensseleitovom otopinom koja je oksigenirana i zagrijana na temperaturu od 36,7 C.<\\\/p>

    Osim u istra\\u017eiva\\u010dke svrhe, sustavi za izolirane organe mogu se koristiti i u nastavne svrhe. Studenti na vje\\u017ebama iz farmakologije mogu \\u201eu\\u017eivo\\u201c vidjeti u\\u010dinke brojnih lijekova na srce i krvne \\u017eile. Tako\\u0111er se mogu pokazivati mehanizmi dijelovanja pojedinih lijekova. Tijekom nastave iz fiziologije studenti su u mogu\\u0107nosti prou\\u010davati promjene na srcu prilikom promjene koncentracije kisika, hranjivih tvari ili elektrolita u otopini kojom se hrani srce.<\\\/p>

    Ovi sustavi predstavljaju novost u Mostaru i Bosni i Hercegovini te daju novu dimenziju znanstveno-istra\\u017eiva\\u010dkom radu na na\\u0161em fakultetu. Tako\\u0111er pru\\u017eaju mogu\\u0107nost studentima za izradu diplomskih radova i lije\\u010dnicima za izvo\\u0111enje pokusa za svoje magisterije i doktorate.<\\\/p>\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"31d1147\"}]},\"elements\":[],\"widgetType\":\"text-editor\"}],\"isInner\":false},{\"id\":\"dehlgfa\",\"elType\":\"section\",\"settings\":{\"layout\":\"full_width\",\"gap\":\"no\",\"content_position\":\"middle\",\"structure\":\"21\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"343907d\"}]},\"elements\":[{\"id\":\"cce1d95\",\"elType\":\"column\",\"settings\":{\"_column_size\":33,\"_inline_size\":30,\"_inline_size_tablet\":100,\"background_background\":\"classic\",\"background_color\":\"#FFFFFF\",\"padding\":{\"unit\":\"%\",\"top\":\"10\",\"right\":\"10\",\"bottom\":\"10\",\"left\":\"25\",\"isLinked\":false},\"padding_tablet\":{\"unit\":\"%\",\"top\":\"10\",\"right\":\"10\",\"bottom\":\"10\",\"left\":\"10\",\"isLinked\":true},\"padding_mobile\":{\"unit\":\"px\",\"top\":\"60\",\"right\":\"30\",\"bottom\":\"30\",\"left\":\"30\",\"isLinked\":false},\"border_border\":\"solid\",\"border_width\":{\"unit\":\"px\",\"top\":\"0\",\"right\":\"1\",\"bottom\":\"0\",\"left\":\"0\",\"isLinked\":false},\"border_color\":\"#F5F5F5\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"c11b5b3\"}]},\"elements\":[{\"id\":\"dc4e96d\",\"elType\":\"widget\",\"settings\":{\"title\":\"\\n\\n\",\"header_size\":\"h4\",\"align\":\"right\",\"align_tablet\":\"center\",\"title_color\":\"#212121\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"d2214da\"}]},\"elements\":[],\"widgetType\":\"heading\"},{\"id\":\"0677353\",\"elType\":\"widget\",\"settings\":{\"title\":\"Suradnja\\n\",\"align\":\"right\",\"align_tablet\":\"center\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"918475c\"}]},\"elements\":[],\"widgetType\":\"heading\"},{\"id\":\"2c69c11\",\"elType\":\"widget\",\"settings\":{\"align\":\"right\",\"align_tablet\":\"center\",\"display_condition_list\":[{\"display_condition_login_status\":\"subscriber\",\"_id\":\"2f6f355\"}],\"editor\":\"

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    Objavljeni radovi zadnjih 5 godina:<\\\/strong><\\\/p>

    1. Unfirer S, Mihalj M, Novak S, Kibel A, Cavka A, MihaljevicZ, GrosM, Brizic I, Budimir D, Cosic A, Boban M, Drenjancevic I. Hyperbaric oxygenation affects the mechanisms of acetylcholine-induced relaxation in diabetic rats. UHM 2016, Vol. 43, No. 6<\\\/li>
    2. Karabuva S, Brizi\\u0107 I, Latinovi\\u0107 Z, Leonardi A, Kri\\u017eaj I, Luk\\u0161i\\u0107 B. Cardiotoxic effects of the Vipera ammodytes ammodytes venom fractions in the isolated perfused rat heart. Toxicon. 2016 Oct;121:98-104. doi: 10.1016\\\/j toxicon. 2016. 09. 001.<\\\/li>
    3. Prskalo Z, Brizi\\u0107 I, Markota D, Markota I, Boban M, Tomic M, Starcevic B. Arterial stiffness in patients with coronary artery disease: relation with in-stent restenosis following percutaneous coronary intervention. BMC Cardiovasc Disord. 2016 Jun 6;16:128. doi: 10.1186\\\/s12872-016-0305-4.<\\\/li>
    4. Karabuva S, Vrki\\u0107 I, Brizi\\u0107 I, Ivi\\u0107 I, Luk\\u0161i\\u0107 B. Venomous snakebites in c hildren in southern Croatia. Toxicon. 2016; pii: S0041-0101(16)30008-3. doi: 10.1016\\\/j.toxicon.2016.01.057.<\\\/li>
    5. Jeroncic A, Gunjaca G, Mrsic DB, Mudnic I, Brizic I, Polasek O, Boban M. Normative equations for central augmentation index: assessment of inter-population applicability and how it could be improved. Sci Rep. 2016 May 27;6:27016. doi: 10.1038\\\/srep27016.<\\\/li>
    6. Markota D, Prskalo Z, Markota I, Starcevic B, Maskovic J, Tomic M, Brizic I: Multiple Multilateral Coronary-Cameral Fistulae in a Patient with Minor Cardiac Venous System. Case Reports in Cardiology. Volume 2014; Article ID 754703, 3 pages\\u00a0http:\\\/\\\/dx.doi.org\\\/10.1155\\\/2014\\\/754703<\\\/a><\\\/li>
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